Importance of experienced thermal history: Effect of acclimation temperatures on the high-temperature tolerance and growth performance of juvenile marbled flounder

Experienced thermal history often affects the temperature tolerance of fish; however, effect on growth performance is unclear. To contribute to effective stocking (release hatchery-reared juveniles in field), we conducted four laboratory experiments using juvenile marbled flounder (Pseudopleuronectes yokohamae, around 30 mm standard length and 0.3 g body wet weight) acclimated at 12 °C 24 for approximately 2 weeks investigate effects acclimation high-temperature tolerance, food consumption, performance. The increased high temperatures, as shown a 24-h exposure experiment elevation experiment. 50% lethal (upper incipient temperature) was estimated be 25.9 29.0 groups, respectively. In subsequent experiments, tested low consumption two size groups (28.7 ± 2.0 34.5 2.9 mm, mean SD), that were reared temperatures ranging from 14 23 °C. optimal 20 did not differ between or groups. However, suppressed by acute changes. Specifically, feeding lower °C-acclimated group than when exposed °C, which close natural water release field. These results suggest experienced does affect but can juveniles. maximize post-release juveniles, influence should taken into consideration changes before avoided.